Absence of light-induced proton extrusion in a cotA-less mutant of Synechocystis sp. strain PCC6803

Size of cotA and identification of the gene product in Synechocystis sp. strain PCC6803.

cotA of Synechocystis sp. strain PCC6803 is a gene involved in light-induced proton extrusion (A. Katoh, M. Sonoda, H. Katoh, and T. Ogawa, J. Bacteriol. 178:5452-5455, 1996). There are two possible initiation codons in cotA, and either long (L-) or short (S-) cotA encoding a protein of 440 or 247 amino acids could be postulated. To determine the gene size, we inserted L-cotA and S-cotA into th...

Multiple light inputs control phototaxis in Synechocystis sp. strain PCC6803.

The phototactic behavior of individual cells of the cyanobacterium Synechocystis sp. strain PCC6803 was studied with a glass slide-based phototaxis assay. Data from fluence rate-response curves and action spectra suggested that there were at least two light input pathways regulating phototaxis. We observed that positive phototaxis in wild-type cells was a low fluence response, with peak spectra...

Phycobilisome assembly in Synechocystis sp. strain PCC6803

Study of Light Wavelength Dependency in Red-Orange Spectrum on Continuous Culture of Synechocystis sp. PCC6803

In this study, the effect of light wavelength on growth rate and lipid production of Synechocystis was investigated. Continuous cultivation system was used to have uniform cell density and avoid self-shading in order to obtain more precise results. Based on previous studies, red light is more efficient than other colors in the visible spectrum for cultivation of Synechocystis; however, the opti...

Quantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803.

BACKGROUND This study aimed at exploring the molecular physiological consequences of a major redirection of carbon flow in so-called cyanobacterial cell factories: quantitative whole-cell proteomics analyses were carried out on two (14)N-labelled Synechocystis mutant strains, relative to their (15)N-labelled wild-type counterpart. Each mutant strain overproduced one specific commodity product, ...